Scientist / Senior Scientist, Multiomics Applications

About this role

About Glyphic:

At Glyphic Biotechnologies, we plan to create the protein revolution for which scientists and researchers have been waiting. We are developing a massively parallel, single-molecule proteome sequencing platform that will transform life science discovery and usher in a new era of insights into human biology and disease. To date, we have raised >$80M from venture partners and non-dilutive grant funding to achieve our vision of next generation proteome sequencing.

What we are looking for in you

We are seeking a technically driven, detail-oriented, and collaborative Scientist or Senior Scientist to apply and optimize sample preparation workflows across complementary DNA sequencing and RNA sequencing workflows. In this role, you will independently execute and troubleshoot established sample preparation protocols across a broad range of biological inputs, generating high-quality data that supports ProSE performance benchmarking and Glyphic's broader multi-omic strategy, which combines ProSE, RNA-seq, DNA-seq, and Ribo-seq to identify neoantigens, biomarkers, and drug targets.

The ideal candidate is comfortable executing and troubleshooting multi-step nucleic acid-based protocols, rapidly adapting established methods to new sample types, and communicating results clearly across multidisciplinary teams. A track record of hands-on sample prep execution across NGS platforms, and comfort operating in high-throughput environments, will be key to making an immediate impact.

This is an in-person position based at our Berkeley, CA location. Depending on where a candidate falls within our requirements, this position will be filled either as a full-time, exempt position with Glyphic or as a full-time contract position

What you’ll do

Sample Preparation & Assay Execution

• Evaluate and process incoming biological sample inputs, including cultured cells, fixed cells, tissue sections, blood, CSF, and Key Opinion Leader (KOL) samples — assessing sample-specific constraints and analytical requirements before entering the workflow.

• Extract and quantify nucleic acids from these samples, applying established extraction methods across diverse matrices.

• Apply and adapt enrichment, depletion, and dynamic-range management protocols to improve input quality for low-abundance targets and optimize NGS sequencing yield.

• Perform core molecular biology reactions — buffer preparation, reagent formulation, enzymatic reactions, gel electrophoresis, PCR/qPCR, hybridization-based assays, and amplification workflows — to prepare samples for downstream analysis.

• Generate sequencing- and analysis-ready outputs: NGS libraries for DNA-seq, RNA-seq, and ribosome profiling across a broad set of platforms.

• Run samples end to end through sequencing, including flow cell loading, instrument setup, and run monitoring.

• Support the generation and sourcing of standard reference materials using cell culture techniques.

Assay Optimization & Data Analysis

• Test protocol variables — reagent concentrations, incubation times, temperature conditions, wash conditions, sample preparation steps, and workflow timing — to optimize assay performance.

• Generate and analyze high-quality experimental data, evaluating assay performance using metrics such as signal intensity, background, reproducibility, specificity, sensitivity, sample quality, and workflow robustness.

• Analyze and interpret large-scale proteomic and genomic datasets using quantitative and statistical approaches.

• Propose and apply in-process controls and analytical tools to build a comprehensive understanding of the factors driving sequencing efficiency across diverse sample and molecule types.

• Collaborate closely with proteomics, assay development, chemistry and data science teams to design experiments, interpret results, and guide project strategy — including cross-platform efforts (ProSE, RNA-seq, DNA-seq, ribosome profiling, and mass spectrometry).

What you need

Required:

• Degree in biochemistry, chemical biology, molecular biology, or an equivalent field; research experience including:

• PhD / MS / BS with 0+ / 2+ / 4+ years of relevant experience (Scientist I)

• PhD / MS / BS with 4+ / 6+ / 8+ years of relevant experience (Senior Scientist)

• Hands-on experience executing sample preparation protocols across nucleic acid-based platforms, generating quantitative readouts across diverse analytical platforms (Illumina, ONT, PacBio, or similar).

• Demonstrated skill preparing and processing diverse biological sample types (cell lines, tissue, blood, or other research/clinical matrices) for downstream genomic analysis.

• Expertise in NGS library preparation methods (WGS/WES, RNA-seq, and/or ribosome profiling), including nucleic acid extraction, quantification, amplification, and QC.

• Proven ability to independently execute the sequencing run itself, including flow cell loading, instrument setup, and monitoring.

• A track record of applying sample preparation or enrichment methods suited for low-abundance targets or limited input material, including cfDNA and other biofluid-derived analytes.

• Working knowledge of germline versus somatic sequence variation and its relevance to sample prep and analysis decisions.

• Strong problem-solving skills and close attention to detail.

• Clear written and verbal communication skills, with the ability to collaborate effectively across diverse scientific and technical backgrounds.

• Ability to thrive in a fast-paced environment with evolving priorities.

Nice to have:

• Experience with nanopore platforms for sequencing or label-free detection.

• Experience with single-cell or spatial proteomics and transcriptomics.

• Familiarity with neoantigen discovery workflows that integrate genomic, transcriptomic, and proteomic evidence.

• Comfort working in R or Python to input experimental results into and navigate established data analysis workflows.

We’re looking for a teammate that:

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